Produksi Bioetanol dari Hidrolisat Ampas Tebu Sistem Selulase B. Subtilis dengan Variasi Waktu dan Jenis Inokulum

Cahyaning Rini Utami, Hapsari Titi Palupi, Ernawati Ernawati

Abstract


Produksi bioetanol menggunakan hidrolisat ampas tebu dengan sistem selulase Bacillus subtilis memiliki keuntungan karena ampas tebu merupakan bahan baku yang melimpah dan murah, serta proses hidrolisis yang efisien dapat mengonversi lignoselulosa menjadi glukosa yang siap difermentasi. Penelitian ini bertujuan untuk mengevaluasi produksi bioetanol dari hidrolisat ampas tebu dengan menggunakan sistem selulase Bacillus subtilis dan variasi waktu fermentasi serta jenis inokulum. Penelitian ini menggunakan Rancangan Acak Kelompok (RAK) dengan dua faktor: waktu fermentasi (24, 48, 72 jam) dan jenis inokulum (Saccharomyces cerevisiae 5% dan ragi tape 5%), masing-masing pengulangan triplo. Karakteristik bioetanol yang dihasilkan dianalisa rendemen, efisiensi fermentasi dan kadar alkohol. Analisis data dilakukan menggunakan uji ANOVA dua arah, dilanjutkan dengan uji Tukey (α=0,05%) untuk menentukan perlakuan terbaik. Hasil penelitian menunjukkan bahwa perlakuan dengan Saccharomyces cerevisiae 5% pada waktu fermentasi 48 jam memberikan hasil terbaik, dengan rendemen bioetanol 122,16 mL/kg dan kadar alkohol 76,8%. Implikasi dari hasil ini menunjukkan bahwa pemilihan inokulum dan waktu fermentasi yang tepat sangat penting untuk memaksimalkan produksi bioetanol dari ampas tebu


Keywords


bioetanol, waktu fermentasi, Saccharomyces cerevisiae, ragi tape

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References


Afedzi, A. E. K., & Parakulsuksatid, P. (2023). Recent advances in process modifications of simultaneous saccharification and fermentation (SSF) of lignocellulosic biomass for bioethanol production. Biocatalysis and Agricultural Biotechnology, 102961.

Aggarwal, N. K., Kumar, N., & Mittal, M. (2022). Bioethanol production: Past and present. Springer Nature.

Ansar, Nazaruddin, Azis, A. D., & Fudholi, A. (2021). Enhancement of bioethanol production from palm sap (Arenga pinnata (Wurmb) Merr) through optimization of Saccharomyces cerevisiae as an inoculum. Journal of Materials Research and Technology, 14, 548–554. https://doi.org/10.1016/j.jmrt.2021.06.085

Arviani, D. N. , H. A. D. , S. W. , U. C. R. (2024). Kimia Organik. Hei Publishing.

Asase, R. V., Okechukwu, Q. N., & Ivantsova, M. N. (2024). Biofuels: present and future. Environment, Development and Sustainability, 1–29. https://doi.org/10.1007/s10668-024-04992-w

Chamidy, H. N., Saripudin, S., & Permanasari, A. R. (2023). Pengaruh waktu, jumlah yeast, dan konsentrasi substrat pada fermentasi limbah kulit nanas menjadi bioetanol skala home industry. Jurnal Serambi Engineering, 8(4). https://doi.org/10.32672/jse.v8i4.6784

Chen, X., Zheng, X., Pei, Y., Chen, W., Lin, Q., Huang, J., Hou, P., Tang, J., & Han, W. (2022). Process design and techno-economic analysis of fuel ethanol production from food waste by enzymatic hydrolysis and fermentation. Bioresource Technology, 363, 127882. https://doi.org/10.1016/j.biortech.2022.127882

da Silva Fernandes, F., de Souza, É. S., Carneiro, L. M., Alves Silva, J. P., de Souza, J. V. B., & da Silva Batista, J. (2022). Current ethanol production requirements for the yeast saccharomyces cerevisiae. International Journal of Microbiology, 2022(1), 1–14. https://doi.org/10.1155/2022/7878830

Hou, J., Zhang, Q., Tian, F., Liu, F., Jiang, J., Qin, J., Wang, H., Wang, J., Chang, S., & Hu, X. (2024). Structure changes of lignin and their effects on enzymatic hydrolysis for bioethanol production: a focus on lignin modification. Journal of Biotechnology, 393, 61–73. https://doi.org/10.1016/j.jbiotec.2024.07.012

Iwuozor, K. O., Emmanuel, S. S., Bello-Hassan, M. T., Emenike, E. C., & Adeniyi, A. G. (2024). Advancements in high gravity fermentation strategies for optimizing ethanol production from sugarcane-based substrates. Sugar Tech, 26(4), 1016–1032. https://doi.org/10.1007/s12355-024-01455-4

Kumari, R., Singh, A., Sharma, R., & Malaviya, P. (2024). Conversion of food waste into energy and value-added products: a review. Environmental Chemistry Letters, 22(4), 1759–1790. https://doi.org/10.1007/s10311-024-01742-2

Malik, W. A., & Javed, S. (2021). Biochemical Characterization of Cellulase From Bacillus subtilis Strain and its Effect on Digestibility and Structural Modifications of Lignocellulose Rich Biomass. Frontiers in Bioengineering and Biotechnology, 9, 800265. https://doi.org/10.3389/fbioe.2021.800265

Mendiburu, A. Z., Lauermann, C. H., Hayashi, T. C., Mariños, D. J., Rodrigues da Costa, R. B., Coronado, C. J. R., Roberts, J. J., & de Carvalho, J. A. (2022). Ethanol as a renewable biofuel: Combustion characteristics and application in engines. Energy, 257, 124688. https://doi.org/10.1016/j.energy.2022.124688

Mohd Azhar, S. H., Abdulla, R., Jambo, S. A., Marbawi, H., Gansau, J. A., Mohd Faik, A. A., & Rodrigues, K. F. (2017a). Yeasts in sustainable bioethanol production: A review. Biochemistry and Biophysics Reports, 10, 52–61. https://doi.org/10.1016/j.bbrep.2017.03.003

Mohd Azhar, S. H., Abdulla, R., Jambo, S. A., Marbawi, H., Gansau, J. A., Mohd Faik, A. A., & Rodrigues, K. F. (2017b). Yeasts in sustainable bioethanol production: A review. Biochemistry and Biophysics Reports, 10, 52–61. https://doi.org/10.1016/j.bbrep.2017.03.003

Nafiqoh, N., & Suryaningrum, L. H. (2020). Hidrolisis ampas tebu menggunakan enzim selulase dari bakteri Bacillus subtilis dalam upaya pemanfaatannya sebagai bahan pakan ikan. Prosiding Seminar Nasional Biologi, 6(1), 428–435. https://doi.org/https://doi.org/10.24252/psb.v6i1.16022

Osman, A. I., Qasim, U., Jamil, F., Al-Muhtaseb, A. H., Jrai, A. A., Al-Riyami, M., Al-Maawali, S., Al-Haj, L., Al-Hinai, A., Al-Abri, M., Inayat, A., Waris, A., Farrell, C., Maksoud, M. I. A. A., & Rooney, D. W. (2021). Bioethanol and biodiesel: Bibliometric mapping, policies and future needs. Renewable and Sustainable Energy Reviews, 152, 111677. https://doi.org/10.1016/j.rser.2021.111677

Parapouli, M., Vasileiadi, A., Afendra, A.-S., & Hatziloukas, E. (2020). Saccharomyces cerevisiae and its industrial applications. AIMS Microbiology, 6(1), 1–32. https://doi.org/10.3934/microbiol.2020001

Pratiwi, I., Miarti, A., Setiorini, I. A., Kurniasari, D., & Kusniawati, E. (2024). Teknik Bioenergi. PT. Sonpedia Publishing Indonesia.

Saisa, & Syabriana, M. (2018). Produksi bioetanol dari limbah kulit kopi menggunakan enzim zymomonas mobilis dan saccharomyces cereviseae. Jurnal Serambi Engineering, 3(1). https://doi.org/10.32672/jse.v3i1.356

Sulistiawan, A., & Nurdiansyah, D. (2022). The development of bioethanol from iles-iles tubers using hydrolyzed fermentation method for alternative fuels. The 4th International Conference on University Community Engagement (ICON-UCE 2022), 4, 40–46. Retrieved from https://icon-uce.com/index.php/icon-uce/article/view/6

Tenkolu, G. A., Kuffi, K. D., & Gindaba, G. T. (2024). Optimization of fermentation condition in bioethanol production from waste potato and product characterization. Biomass Conversion and Biorefinery, 14(4), 5205–5223. https://doi.org/10.1007/s13399-022-02974-4

Thangavelu, S. K., Ahmed, A. S., & Ani, F. N. (2016). Corrosive characteristics of bioethanol and gasoline blends for metals. International Journal of Energy Research, 40(12), 1704–1711. https://doi.org/10.1002/er.3555

Tuli, D. K., & Kuila, A. (2021). Current status and future scope of microbial cellulases. Elsevier.

ul-Haque, Dr. S. N. S. M., & Mueedin, N. (2021). Fermentation of tapai and alcohol content released from tapai. IOP Conference Series: Materials Science and Engineering, 1053(1), 012050. https://doi.org/10.1088/1757-899X/1053/1/012050

Umar, A., Usman, N., Wali, M., & Augustine, O. (2024). Assessment and characterisation of bacillus subtilis as a potential probiotic agent for cellulose digestion. European Journal of Scientific Research and Reviews, 0, 1. https://doi.org/10.5455/EJSRR.20240823083355

Utami, C. R. (2017). Pengaruh waktu fermentasi terhadap karaktersitik kimia dan organoleptik tape pisang kepok. TEKNOLOGI PANGAN: Media Informasi Dan Komunikasi Ilmiah Teknologi Pertanian, 8(1), 99–106. https://doi.org/10.35891/tp.v8i1.904

Zhuang, S., Renault, N., & Archer, I. (2021). A brief review on recent development of multidisciplinary engineering in fermentation of Saccharomyces cerevisiae. Journal of Biotechnology, 339, 32–41. https://doi.org/10.1016/j.jbiotec.2021.07.013




DOI: https://doi.org/10.54256/isrj.v4i2.130

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