Assessement of Glifosat herbicide to viability of decomposer B Com T isolate in vitro

Donny Nugroho Kalbuadi, Muhammad Abdul Azis, Aris Lukito, Lydia Ayu Utami, Salsa Bila Alzahra, Khansa Destiandani, Happy Widiastuti

Abstract


Sugarcane plantation produces approximately 10 to 12 tons of trash per hectare in a single growing season. The high content of lignin and cellulose in the litter necessitates the use of decomposers to accelerate the decomposition process, thereby enhancing the benefits of trash application to soil fertility and plant growth. However, decomposers are microorganisms whose effectiveness is influenced by their viability in the field. In sugarcane land management practices, herbicide application is performed to control weeds during the 1-2 month growth phase of sugarcane/ratoon. This study aimed to evaluate the in vitro resistance of the B-Comp T decomposer isolate to glyphosate herbicide. B-Comp T is a microbial consortium comprising one cellulolytic fungus (Trichoderma sp.), two lignolytic fungi (LGT1 and Omphalina sp), one lignolytic bacteria (LT5), and two cellulolytic bacteria (S sereh and SPC 5). The experiment was conducted using Petri dishes with PDA medium for fungi and NA medium for bacteria. Glyphosate herbicide concentrations tested were 0%, 0.03%, 0.06%, 0.12%, and 0.15%, which were incorporated into the medium during sterile medium pouring into Petri dishes. Fungi incubation was carried out for 7 days while 5 days for bacteria. Microbial growth was observed visually. The observation results demonstrated that the effect of glyphosate on microbial growth varied among species, as did their tolerance limits. At low concentrations, glyphosate generally stimulated microbial growth, whereas at high concentrations, it inhibited microbial growth. Among the tested decomposer isolates, the bacterial isolates LPC 2 and SPC 5 exhibited resistance to glyphosate, while the most susceptible isolate was lignolytic wite rot fungi Omphalina sp and cellulolytic bacteria S. sere. 

Keywords


Sugarcane litter decomposer; lignolytic; cellulolytic; consortium

Full Text:

PDF

References


Baldrian, P., Valásková, V., Merhautová, V., & Gabriel, J. (2005). Degradation of lignocellulose by Pleurotus ostreatus in the presence of copper, manganese, lead and zinc. Research in microbiology, 156(5-6), 670–676. https://doi.org/10.1016/j.resmic.2005.03.007.

Borggaard, O. K., & Gimsing, A. L. (2008). Fate of glyphosate in soil and the possibility of leaching to ground and surface waters: a review. Pest management science, 64(4), 441–456. https://doi.org/10.1002/ps.1512.

Castillo, M.delP., Torstensson, L., & Stenström, J. (2008). Biobeds for environmental protection from pesticide use--a review. Journal of agricultural and food chemistry, 56(15), 6206–6219. https://doi.org/10.1021/jf800844x.

Duke S. O. (2018). The history and current status of glyphosate. Pest management science, 74(5), 1027–1034. https://doi.org/10.1002/ps.4652.

Haney, R. L., Senseman, S. A, Hons, F.M., & Zuberer, D. (2009). Effect of glyphosate on soil microbial activity and biomass. Weed Science, 48, 89–93.

Kapich, A., Hofrichter, M., Vares, T., & Hatakka, A. (1999). Coupling of manganese peroxidase-mediated lipid peroxidation with destruction of nonphenolic lignin model compounds and 14C-labeled lignins. Biochemical and biophysical research communications, 259(1), 212–219. https://doi.org/10.1006/bbrc.1999.0742.

Kepler, R. M., Epp Schmidt, D. J., Yarwood, S. A., Cavigelli, M. A., Reddy, K. N., Duke, S. O., Bradley, C. A., Williams, M. M., II, Buyer, J. S., & Maul, J. E. (2020). Soil microbial communities in diverse agroecosystems exposed to the herbicide glyphosate. Applied and environmental microbiology, 86(5), 1744–1819. https://doi.org/10.1128/aem.01744-19.

Kolpin, D. W., Thurman, E. M., Lee, E. A., Meyer, M. T., Furlong, E. T., & Glassmeyer, S. T. (2006). Urban contributions of glyphosate and its degradate AMPA to streams in the United States. The Science of the total environment, 354(2-3), 191–197. https://doi.org/10.1016/j.scitotenv.2005.01.028.

Kunamneni, A., Ballesteros, A., Plou, F. J., & Alcalde, M. (2007). Fungal laccase: A versatile enzyme for biotechnological applications. In A. Méndez-Vilas (Ed.), Communicating current research and educational topics and trends in applied microbiology (pp. 233–245). Formatex.

Leonowicz, A., Cho, N. S., Luterek, J., Wilkolazka, A., Wojtas-Wasilewska, M., Matuszewska, A., Hofrichter, M., Wesenberg, D., & Rogalski, J. (2001). Fungal laccase: properties and activity on lignin. Journal of basic microbiology, 41(3-4), 185–227. https://doi.org/10.1002/1521-4028(200107)41:3/4<185::aid-jobm185>3.0.co;2-t.

Morozova, O. V., Shumakovich, G. P., Shleev, S. V., & Iaropolov, A. I. (2007). Prikladnaia biokhimiia i mikrobiologiia, 43(5), 583–597.

Pizzul, L., Castillo, M.delP., & Stenström, J. (2009). Degradation of glyphosate and other pesticides by ligninolytic enzymes. Biodegradation, 20(6), 751–759. https://doi.org/10.1007/s10532-009-9263-1.

Prashar, P., Kapoor, N., & Sachdeva, S. (2014). Rhizosphere: its structure, bacterial diversity and significance. Rev Environ Sci Biotechnol, 13, 63–77. https://doi.org/10.1007/s11157-013-9317-z.

Rueda-Ruzafa, L., Cruz, F., Roman, P., & Cardona, D. (2019). Gut microbiota and neurological effects of glyphosate. Neurotoxicology, 75, 1–8. https://doi.org/10.1016/j.neuro.2019.08.006.

Sibalekile, A., Araya, T., Castillo Hernandez, J., & Kotzé, E. (2025). Glyphosate-microbial interactions: metagenomic insights and future directions. Frontiers in microbiology, 16, 1570235. https://doi.org/10.3389/fmicb.2025.1570235.

Souza, K. S., da Silva, M. R. F., Candido, M. A., Lins, H. T. S., de Lima Torres, G., da Silva Felix, K. C., Silva, K. C. C., Filho, R. M. N., Bhadouria, R., Tripathi, S., Singh, R., Santos, M. D. V., Silva, I. P. S., de Barros, A. V., de Araújo, L. C. A., Motteran, F., & de Oliveira, M. B. M. (2025). Biodegradation potential of glyphosate by bacteria: a systematic review on metabolic mechanisms and application strategies. Agronomy, 15(5), 1247. https://doi.org/10.3390/agronomy15051247.

Spinelli, V., Ceci, A., Dal Bosco, C., Gentili, A., & Persiani, A. M. (2021). Glyphosate-Eating Fungi: Study on Fungal Saprotrophic Strains' Ability to Tolerate and Utilise Glyphosate as a Nutritional Source and on the Ability of Purpureocillium lilacinum to Degrade It. Microorganisms, 9(11), 2179. https://doi.org/10.3390/microorganisms9112179.

Stoilova, I., Krastanov, A., & Stanchev, V. (2010). Properties of crude laccase from Trametes versicolor produced by solid-substrate fermentation. Advances in Bioscience and Biotechnology, 1, 208–215.

Torstensson, L. (1985). Behaviour of glyphosate in soils and its degradation. In E. Grossbard & D. Atkinson (Eds.), The herbicide glyphosate (pp. 137–150). Butterworths.

van Bruggen, A. H. C., Goss, E. M., Havelaar, A., van Diepeningen, A. D., Finckh, M. R., & Morris, J. G., Jr (2019). One Health - Cycling of diverse microbial communities as a connecting force for soil, plant, animal, human and ecosystem health. The Science of the total environment, 664, 927–937. https://doi.org/10.1016/j.scitotenv.2019.02.091.

Vázquez, M. B., Moreno, M. V., Amodeo, M. R., & Bianchinotti, M. V. (2021). Effects of glyphosate on soil fungal communities: A field study. Revista Argentina de microbiologia, 53(4), 349–358. https://doi.org/10.1016/j.ram.2020.10.005.




DOI: https://doi.org/10.54256/isrj.v6i1.162

Refbacks

  • There are currently no refbacks.


Copyright (c) 2026 Donny Nugroho Kalbuadi, Muhammad Abdul Azis, Aris Lukito, Lydia Ayu Utami, Salsa Bila Alzahra, Khansa Destiandani, Happy Widiastuti

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Editorial Office:
Pusat Penelitian Perkebunan Gula Indonesia
Jl.Pahlawan Nomor 25 Pasuruan 67126, Indonesia
Telp:+62 0343 421086 ; Fax: +62 0343 421178
Email: p3gipasuruanok@gmail.com; web perusahaan:http://www.p3gi.co.id

Creative Commons License
Indonesian Sugar Research Journal (ISRJ) is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.